Journal: Annals of Translational Medicine

Article Title: The landscape of m6A regulators in esophageal cancer: molecular characteristics, immuno-oncology features, and clinical relevance

doi: 10.21037/atm-22-5895

Figure Lengend Snippet: In vivo and in vitro functional assay involving VIRMA . (A) Western blot analysis of VIRMA expression efficiency in siRNA knockdown KYSE410 and OE19 cells. (B) The CCK-8 assay was used to detect the viability of KYSE410 and OE19 cells transfected with VIRMA siRNAs or control. (C) Western blot analysis of VIRMA expression efficiency in shRNA knockdown KYSE410 and OE19 cells. (D) The Transwell assay was performed to detect the migration and invasion ability of KYSE410 and OE19 cells transfected with VIRMA siRNAs or control. Stained with 3% crystal violet in methanol. (E) Subcutaneous tumorigenicity assay of KYSE410 and OE19 cells transfected with VIRMA shRNA or control, the right side is the H&E staining of the tumor. Data are representative of at least three independent experiments. Data are presented as mean ± SEM. ****, P<0.0001. NC, negative control; H&E, hematoxylin and eosin; SEM, standard error of the mean.

Article Snippet: Each day, one 96-well plate was used, and 10 µL/well of Cell Counting Kit-8 (CCK-8) (Bioss, Beijing, China) solution was added.

Techniques: In Vivo, In Vitro, Functional Assay, Western Blot, Expressing, CCK-8 Assay, Transfection, shRNA, Transwell Assay, Migration, Staining, Tumorigenicity Assay, Negative Control

Journal: International Journal of Medical Sciences

Article Title: Identification of an Anoikis-associated LncRNA Signature to Predict the Clinical Prognosis and Immune Function of Patients with Endometrial Cancer

doi: 10.7150/ijms.107243

Figure Lengend Snippet: (A) KM Plotter database exhibited the relationship between expression of CFAP58-DT and OS in EC patients. (B) qRT-PCR showed the CFAP59-DT expression in different EC cells. (C-H) CCK8, EdU, Calcein/PI cell staining, flow cytometry and colony formation assays showed shCFAP59-DT enhanced the apoptosis and inhibited proliferation of KLE cells. (I) Western blot showed the protein levels of BAX, Bcl2, Caspase3, Active Caspase3.β-Actin is an internal parameter.

Article Snippet: After incubation for 24 h, the medium in each well was replaced with fresh medium containing 10% CCK8 (ABclonal, China) and incubated for 1-2 h in the dark at a constant temperature.

Techniques: Expressing, Quantitative RT-PCR, Staining, Flow Cytometry, Western Blot